Mechanistic Approach to the Problem of Hybridization Efficiency in Fluorescent In Situ Hybridization
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چکیده
منابع مشابه
Mechanistic approach to the problem of hybridization efficiency in fluorescent in situ hybridization.
In fluorescent in situ hybridization (FISH), the efficiency of hybridization between the DNA probe and the rRNA has been related to the accessibility of the rRNA when ribosome content and cell permeability are not limiting. Published rRNA accessibility maps show that probe brightness is sensitive to the organism being hybridized and the exact location of the target site and, hence, it is highly...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2004
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.70.12.7126-7139.2004